A Review on Bioanalytical Method Development and Validation for Estimation of Drugs by Using Hyphenated Techniques and its Applications on Routine Analysis

Bioanalytical methods are used to create a protocol that can identify and quantify bioactive molecules their metabolites in human animal samples. For determining the medication its present human/animal body, bioanalytical method is efficient. One of prominent roles creating new techniques, validating existing processes, analyzing Most importantly, compound be calculated using many also detected various analytical methods. In specific biological samples, drugs evaluated by different extraction procedures, such as solid-phase extraction, liquid protein precipitation etc.., All steps were investigated know how environment, sample, or techniques influence matrix assessment up study's time. The methods, including high-pressure (HPLC), chromatography combined with dual-mass spectroscopy (LCMS-MS), Ultra-performance (UPLC), obtain in-depth analysis medications. have strengths weaknesses. Currently, bioanalysis usually done HPLC-MS GCMS-MS. Linearity, repeatability, Accuracy, precision, selectivity, recovery consistency essential criteria. this review we proposed give importance system production validation parameters aid drug quality, purity, safety efficacy.